Resazurin Assay Applications Diagram

This file contains a visual representation of how the resazurin assay can be applied across the aquaculture industry.

Simple Flow Diagram

RESAZURIN ASSAY: Measuring Oyster Health & Stress Tolerance

                           OYSTER SAMPLE
                                │
                                ▼
                        ┌──────────────────┐
                        │  RESAZURIN ASSAY │
                        │                  │
                        │ • Add dye        │
                        │ • Apply stress   │
                        │ • Measure signal │
                        └─────────┬────────┘
                                  │
                                  ▼
                          METABOLIC ACTIVITY
                         (Fluorescence Signal)
                                  │
                    ┌─────────────┼─────────────┐
                    ▼             ▼             ▼
               HIGH SIGNAL   MEDIUM SIGNAL   LOW/NO SIGNAL
              (Stressed but  (Healthy &      (Stressed &
               still active)  active)        shutting down)
                    │             │             │
                    ▼             ▼             ▼
               POOR SURVIVAL  GOOD HEALTH   GOOD SURVIVAL
               (Dies quickly) (Thriving)    (Conserving energy)

APPLICATIONS:

┌─────────────────────────────────────────────────────────────────────┐
│                          BREEDING PROGRAMS                          │
├─────────────────────────────────────────────────────────────────────┤
│ • Select parents with good stress responses                         │
│ • Identify heat-tolerant genetic lines                             │
│ • Accelerate genetic improvement                                    │
└─────────────────────────────────────────────────────────────────────┘

┌─────────────────────────────────────────────────────────────────────┐
│                         FARM MANAGEMENT                             │
├─────────────────────────────────────────────────────────────────────┤
│ • Test environmental conditions                                     │
│ • Monitor oyster health during stressful periods                   │
│ • Optimize placement and timing of harvests                        │
└─────────────────────────────────────────────────────────────────────┘

┌─────────────────────────────────────────────────────────────────────┐
│                      RESEARCH & DEVELOPMENT                         │
├─────────────────────────────────────────────────────────────────────┤
│ • Study climate change impacts                                      │
│ • Test new farming techniques                                       │
│ • Understand stress physiology                                      │
└─────────────────────────────────────────────────────────────────────┘

Industry Impact Timeline

IMMEDIATE (0-2 years)        NEAR-TERM (2-5 years)        LONG-TERM (5+ years)
     │                            │                            │
     ▼                            ▼                            ▼
┌──────────┐                ┌──────────┐                ┌──────────┐
│Research  │                │Commercial│                │Industry  │
│adoption  │                │services  │                │standard  │
│          │                │available │                │practice  │
└──────────┘                └──────────┘                └──────────┘
     │                            │                            │
     ▼                            ▼                            ▼
• Lab protocols            • Hatchery testing          • Routine screening
• Validation studies       • Breeding programs         • Climate adaptation
• Pilot projects          • Farm consultations        • Global implementation